|Holbrook, Carl - Corley|
Submitted to: American Peanut Research and Education Society Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 7/1/2007
Publication Date: 7/14/2007
Citation: Guo, B., Dang, P.M., Li, Y., Chen, A.K., Culbreath, A.C., Holbrook, Jr., C.C. 2007. Development of peanut expressed sequence tag-based genomic resources and tools [abstract]. Proceedings of the American Peanut Research and Education Society Annual Meeting, July 10-13, 2007, Birmingham, Alabama. p. 59.
Technical Abstract: U.S. Peanut Genome Initiative (PGI) has widely recognized the need for peanut genome tools and resources development for mitigating peanut allergens and food safety. Genomics such as Expressed Sequence Tag (EST), microarray technologies, and whole genome sequencing provides robotic tools for profiling genes. In spite of continuous decrease in DNA sequencing costs, it is improbable that many large plant genomes, such as peanut, will be sequenced in the near future. However, partially sequencing of large numbers of expressed genes (ESTs) can deliver substantial amounts of genetic information that will allow comparative and functional studies. Notable research progress has been made recently in development of peanut ESTs. Up to today, we have sequenced a total of 44,064 cDNA clones from ten peanut cDNA libraries. After comparison and assembly of overlapping sequences, about 10,096 unique sequences have been identified. This sequence data will be available to the community in order to develop genomic tools and resources for deciphering the chromosomal location and biological function of genes in the peanut genome and mitigating peanut food safety issues. Our interests are two fold: construction of peanut 70-mer oligo microarray consisting over 10,000 gene-elements in collaboration with TIGR (the Institute for Genomic Research) and development of markers/genes associated with disease resistance, such as TSWV and leaf spots. A panel of 16 diverse peanut genotypes has been screened for genetic diversity. Several RIL (recombinant inbred line) populations have been constructed for advancement.