Submitted to: Plant and Animal Genome Conference Proceedings
Publication Type: Abstract only
Publication Acceptance Date: 10/15/2006
Publication Date: 1/14/2007
Citation: Figueroa, D.M., Amarillo, F.E., Ferdette, N.C., Morganti, A.T., Davis, J.D., Lawrence, C.J., Bass, H.W. 2007. Constructing a Cytogenetic Map of the Maize Genome. In: Plant and Animal Genome Conference Proceedings. Annual International Plant and Animal Genome Conference, January 13-17, 2007, San Diego, CA. XV:P349. Interpretive Summary:
Technical Abstract: We are developing a pachytene cytogenetic FISH (Fluorescence in situ Hybridization) map of the maize (Zea mays L.) genome using maize marker-selected sorghum BACs (Bacterial Artificial Chromosome) as described by Koumbaris and Bass (2003, Plant J. 35:647). The two main projects are the production of a high-density map of chromosome 9 and a medium resolution map of the other chromosomes using the maize Core Bin Marker (CBM) loci from the UMC98 maize linkage map. Analysis of 30 FISH-mapped loci on chromosome 9 revealed colinearity between the cytogenetic and linkage maps, with localization of the centromere between tda66d and cdo17. Interestingly, we found chromosomal regions that appear to be hotspots of genome expansion (e.g., 9L.55-9L.72). For the CBM mapping project, 47 of the 90 CBM probes have been subjected to sorghum BAC library screening. Analysis of chromosome addition lines 4 and 6 revealed a preservation of the arm ratios (Long/Short = 1.6±0.29 for 4, 2.7±0.38 for 6). In addition, we are obtaining full-length insert sequence data for many of the RFLP (Restriction Fragment Length Polymorphism) probes used in this project (www.plantgdb.org/prj/RFLP_FLIS/). A local summer outreach project, the Maize-10-Maze project, was carried out using a living field replica of the maize genome with the 10 maize chromosomes represented by 10 rows of corn. Data and details of these projects are available online at www.cytomaize.org and www.maizegdb.org.