|Rinehart, Timothy - Tim|
Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 7/7/2007
Publication Date: 7/7/2008
Citation: Wang, X., Trigiano, R., Windham, M., Scheffler, B.E., Rinehart, T.A., Spiers, J.M. 2008. Microsatellites and Their Appliation in Flowering Dogwood. Meeting Abstract.
Technical Abstract: Microsatellites, also known as simple sequence repeats (SSRs), are repeat units that are 1 to 6 base pairs long and repeat six or more times and are present throughout the entire eukaryotic genome. SSRs are useful genetic markers for studying genetic diversity and for creating linkage maps of plants. Flowering dogwood (C. florida L.) is a popular ornamental species in North America, Asia and Europe and there are over 100 named cultivars to date. We constructed four SSR-enriched small insert genomic libraries for flowering dogwood using a modified existing microsatellite-enrichment protocol and a new SSR detecting method. An average of 46% of SSR enrichment in the four libraries (66% in AC library, 62% in AG library, 30% in AAC library and 28% in ATG library) was obtained and a total of 780 positives clones have been detected by a simple PCR method contained SSR motifs. A total of 351 SSR primer pairs were designed from these positive sequences. About 89% of these primers amplified polymorphic loci among 56 flowering dogwood cultivars. Twenty-five SSR primer pairs selected from 351 SSR primers detected a total of 195 alleles. The total diversity was 0.926 in the 56 flowering dogwood cultivars, indicating a high level of genetic variation among these selected cultivars. About 58% of these primers showed polymorphism between two flowering dogwood accessions (95-10 and 95-1). These primers will be used for an F1 mapping population between 95-10 and 95-1 and try to generate an initial parent linkage map in flowering dogwood. Our results indicate that first SSR-enriched libraries in C. florida has been obtained and applied in a diversity study for flowering dogwood cultivars.