|Bruckart, William - Bill|
Submitted to: Phytopathology
Publication Type: Abstract Only
Publication Acceptance Date: 3/21/2007
Publication Date: 7/1/2008
Citation: Eskandari, F., Bruckart, W.L. 2008. Crosses between pycnia of Puccinia acroptili from the United States, Russia, and Turkey. Phytopathology. 97 (7, Supplement):S184
Technical Abstract: Russian knapweed (Acroptilon repens) is infected both in the United States (U.S.) and in its native range of Eurasia by Puccinia acroptili, a candidate for biological control in the U.S. Small, consistent differences in teliospore length and ITS sequences were found between U.S. and a Turkish isolate in earlier studies. Research to clarify taxonomic relationships of these isolates included artificial crosses. When flecks developed after foliar inoculations with teliospores, leaves were detached and grown in sterile tap water in insect exclusion cages. Pycniospores from a single pycnium were suspended in sterile distilled water and a 1 microliter drop placed on each of the remaining pycnia. Successful reciprocal crosses have been achieved between the Turkish isolate (02-048) and one isolate each from the U.S. (05-055) and Russia (05-085). One-way crosses between a U.S. isolate (05-056) and isolates from Turkey and Russia also were successful. These results suggest that isolates are the same species, despite differences noted earlier. Classical and molecular characterization of isolates from these and subsequent crosses is in progress.