Submitted to: Congress on In Vitro Biology
Publication Type: Abstract Only
Publication Acceptance Date: 4/27/2007
Publication Date: 6/16/2007
Citation: Stanley, D.W., Goodman, C.L., Song, Q., An, S., Mcintosh, A.H. 2007. Prostaglandins A1 and E1 influence gene expression in an established insect cell line (BCIRL-HzAM1) [abstract]. Congress on In Vitro Cellular and Developmental Biology. 43:534.
Technical Abstract: In work to determine the biochemical mechanisms of prostaglandin (PG) action in insect cells, we posed the hypothesis that prostaglandins (PGs) influence gene expression. In separate experiments, we exposed the BCIRL-HzAM1 cell line (derived from pupal ovarian tissue of the cotton bollworm, Helicoverpa zea) to PGA1 (interacts with perinuclear receptors) and PGE1 (acts via G protein coupled receptors). After either 12 or 24 hr exposures to the PGs, control and treated cells were subjected to 2D electrophoresis. Changes in protein expression were recorded by densitometry, showing quantitative changes in >30 proteins. Selected proteins were analyzed by MS/MS (MALDI TOF/TOF). The derived sequences were used in bioinformatic analyses to identify the proteins. Quantitative changes in a subset of proteins were confirmed by RT-PCR. PG treatments influenced expression of genes encoding proteins involved in stress responses or detoxification reactions (e.g., heat shock proteins, Mn superoxide dismutase and catalase). Changes were also recorded in expression of genes encoding proteins acting in cell structure (e.g., actin-depolymerizing factor 1), metabolism (e.g., glyceraldehyde-3-phosphate dehydrogenase) and responses to external stimuli (e.g., tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein). These findings support our hypothesis that both PGA1 and PGE1 influence gene expression in insect cells, as seen in mammalian cells. These two PGs presumably act via different intracellular signal transduction pathways.