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Title: Development of a genetic linkage map for tetraploid highbush blueberry using SSR and EST-PCR markers

Author
item BREVIS, PATRICIO
item HANCOCK, JAMES
item Rowland, Lisa

Submitted to: HortScience
Publication Type: Abstract Only
Publication Acceptance Date: 3/14/2007
Publication Date: 7/1/2007
Citation: Brevis, P., Hancock, J., Rowland, L.J. 2007. Development of a genetic linkage map for tetraploid highbush blueberry using SSR and EST-PCR markers. HortScience, v. 42, p. 963

Interpretive Summary:

Technical Abstract: In an effort to better understand the genetic control of chilling requirement in tetraploid highbush blueberry (Vaccinium corymbosum L.), a genetic linkage map is being constructed from the cross between ‘Draper’ and ‘Jewel’ blueberry cultivars (northern and southern adapted, respectively). The mapping population consists of 105 F1 individuals. SSR markers used in this study were derived from EST libraries constructed from cold acclimated and nonacclimated floral buds, and from a microsatellite-enriched genomic library. From a total of 49 SSR primer pairs tested, 33 amplified segregating amplicons (111 markers). Genetic mapping in polyploids requires the use of informative markers that are Single Dose Restriction Fragments (SDRFs) in one or both parents (segregating 1:1 or 3:1, respectively). Thirty primer pairs (61%) were informative, yielding 72 SDRFs. Also, 17 non-informative markers exhibited segregation ratios consistent with tetrasomic inheritance (5:1, 11:1 or 35:1). The preliminary linkage maps consist of 9 linkage groups for the ‘Draper’ parent (19 SSRs) and 5 for the ‘Jewel’ parent (11 SSRs). Efforts are underway to screen EST-PCR and additional SSR markers for amplification and segregation in the mapping population.