Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 3/13/2007
Publication Date: 5/15/2007
Citation: Siragusa, G.R., Svetoch, E.A., Eruslanov, B.V., Perelygin, V.V., Mitsevich, E.V., Mitsevich, I.P., Levchuk, V.P., Svetoch, O.E., Seal, B.S., Stern, N.J. 2007. A poultry-intestinal isolate of Campylobacter jejuni produces a bacteriocin (CUV-3) active against a range of Gram positive bacterial pathogens including Clostridium perfringens. Meeting Abstract. P. A13, Abstract #48. Interpretive Summary:
Technical Abstract: A newly isolated bacteriocin, CUV-3, produced by a poultry cecal isolate of Campylobacter jejuni strain CUV-3 had inhibitory activity against several Gram positive bacteria including Clostridium perfringens (38 strains), Staphylococcus aureus, Staph.epidermidis and Listeria monocytogenes. The peptide was purifed to homogeneity by chromotography. Based on amino acid sequence and mass spectomtery, the CUV-3 was 2,368 Da, 37 amino acids in length that contained Dha and MeLan residues. NCBI-BLAST indicated CUV-3 contained amino acid sequences that were 66% identical to portions of hemolysins predicted for Sulfitobacter sp., Roseovarius sp. and Jannaschia sp. of the alpha-proteobacteria. CUV-3 was sensitive to proteases and heat resistant (100 deg. C, 15 min) as well as resistant to lysozyme or lipase digestions. Minimum inhibitory concentrations (MIC’s) of purified CUV-3 peptide ranged from <1 to 6.5 ug/ml for the Gram positive species and from 15 to 62 ug/ml for Gram negative species tested. A Campylobacter jejuni non-producing test strain was resistant to the purified bacteriocin (MIC = 100 ug/ml). CUV-3 anti-C. perfringens MIC’s were comparable to that of conventional antibiotics (average MIC in ug/ml against four representative strains): chloramphenicol (17.6) vancomycin (0.3), erythromycin (4.4), imipenem (0.5), tetracycline (0.3), piperacillin (0.1), clindamycin (2.2), CUV-3 (0.3). To our knowledge, this is the first such activity reported for this taxon.