Submitted to: DNA Research
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 7/30/2007
Publication Date: 6/1/2008
Citation: Shultz, J.L., Ray, J.D., Smith, J.R. 2008. Mapping Two Genes in the Purine Metabolism Pathway of Soybean.. DNA Research. 19(3):264-269 Interpretive Summary: Soybean is a primary source of protein for humans and animals. Formation of protein in soybean involves use of nitrogen from the air, and this process is sensitive to even short-term drought stress. The purine pathway plays a dominant role in the use of atmospheric nitrogen in the formation of proteins. Molecular differences between two soybean lines involving two genes controlling the purine pathway were identified. This allowed the location of the two genes in the soybean molecular organization. Determination of the location of the genes is the first step in understanding this biochemical pathway. This information may lead scientists to a better understanding of how drought affects soybean plants and allow the plant to be bred for tolerance to this stress.
Technical Abstract: Mapping genes in biochemical pathways allow study of the genomic organization of pathways and geneic relationships within these pathways. Additionally, molecular markers located within the boundaries of a specific gene sequence represent important marker assisted selection resources. We report map locations of two geneic markers from the purine synthesis pathway in soybean (Glycine max (L. merr.)), utilizing a 90 plant F2 population created from the cross of ‘DT97-4290’ x ‘DS97-84-1’. Primers were designed based on sequences from annotated soybean complimentary DNA (cDNA). A polymorphic, co-dominant, sequence-characterized amplified region (SCAR) marker was created for hypoxanthine phosphoribosyl transferase (E.C. 22.214.171.124). Linkage analysis placed this gene on linkage group (LG) O. In addition, a single-nucleotide polymorphism (SNP) marker was developed for a urate oxidase gene (E.C. 126.96.36.199). Linkage analysis of the SNP placed the urate oxidase gene on LG I. For both genes, amplicon sequence data confirmed the identification of the respective gene. Mapping these genes represent the first step in understanding the genomic organization of the purine biochemical pathway in soybean.