Submitted to: Plant and Animal Genome VX Conference Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 1/1/2007
Publication Date: 1/17/2007
Citation: Iniquez-Luv, F.L., Lukens, L., Farnham, M.W., Amasio, R., Osborn, T.C. 2007. Development of Public Immortal Mapping Populations, Molecular Markers, and Linkage Maps for Rapid Cycling Brassica rapa and B. oleracea. Plant and Animal Genome VX Conference Abstracts. p. 18
Technical Abstract: Past research efforts on genetic mapping in Brassica oleracea and Brassica rapa have been disconnected, utilizing separate mapping populations and different sets of molecular markers. Here we present public immortal mapping populations, molecular markers and linkage maps for rapid cycling B. rapa and B. oleracea. The B. rapa population consists of 150 recombinant inbred (RI) lines derived from a cross of two highly inbred annual lines, rapid cycling IMB211 and yellow sarson R500. The B. oleracea population consists of 150 double haploid (DH) lines derived from the F1 between two DH lines, rapid cycling TO1000DH3 and broccoli line Early Big. A total of 120 RFLP probes, 146 SSR markers (mainly developed in our laboratory), and one phenotypic trait (flower color) were used to construct genetic maps for both species. The RI B. rapa genetic map consists of 224 molecular markers distributed along 10 linkage groups (R1-R10 standard nomenclature) with a total distance of 1125.3 cM and a markers density of 5.7 cM/marker. The DH B. oleracea genetic map consists of 279 molecular markers and one phenotypic marker distributed along 9 linkage groups (O1-O9 standard nomenclature) with a total distance of 891.4 cM and a markers density of 3.2 cM/marker. A syntenic analysis with Arabidopsis thaliana was conducted for each of the two genetic maps. The use of these maps as tools for the Brassica research community will be described, with especial emphasis on bulk segregant mapping of B. oleracea EMS mutants.