Submitted to: Planta
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 6/25/2007
Publication Date: 6/25/2007
Citation: Klink, V.P., Overall, C., Macdonald, M.H., Matthews, B.F. 2007. A time-course comparative microarray analysis of an incompatible and compatible response by Glycine max (soybean) to Heterodera glycines (soybean cyst nematode) infection. Planta. 226:1423-1447. Interpretive Summary: The soybean cyst nematode causes almost one billion dollars in losses to the soybean crop each year in the USA. Better understanding of the resistance and susceptible responses of soybean to nematodes may provide new approaches to developing nematode-resistant soybean. We analyzed the expression of over 35,000 soybean genes in roots susceptible and resistant to the soybean cyst nematode and identified numerous genes with minor differences in their level of expression at 12 hours, 3 days and 8 days post-infection by the nematode. This information will be useful to scientists working to improve resistance of plants to nematodes using biotechnology and engineering.
Technical Abstract: The development of infection in soybean cv. Peking roots by compatible or incompatible races of Heterodera glycines (soybean cyst nematode [SCN]) was assayed by microarray analyses. Using three time-points, one as nematodes penetrate the root prior to feeding site selection; a second as the nematode develops its site of nutrient intake (syncytium); and a third as the nematodes actively ingest nutriment from the syncytium, allowed for sorting differentially expressed genes into expression profiles. Consequently, those analyses allowed for the identification of signature gene expression patterns for each outcome for both G. max and H. glycines. We identified 3251 differentially expressed genes that are common to both the developing compatible and incompatible infection processes. Thus, these genes may represent a core set of genes that are differentially expressed irrespective of the compatible or incompatible G. max response to H. glycines. We identified 775 differentially expressed H. glycines genes at 12 hr post inoculation demonstrating intrinsic differences between the compatible and incompatible races prior to feeding site selection. Syncytia were collected by laser capture microdissection (LCM) and also analyzed using microarrays. These data provide a better understanding of the complex response of plants to nematodes in susceptible and resistant interactions.