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United States Department of Agriculture

Agricultural Research Service

Title: The Neonatal calf Tuberculosis Vaccine Model: Immune Responses to Protective and Non-protective Vaccines after Aerosol Challenge with Virulent Mycobacterium bovis)

Author
item Waters, Wade
item Palmer, Mitchell
item Nonnecke, Brian
item Thacker, Tyler
item Estes, Mark
item Jacobs, William
item Larsen, Michelle

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 5/19/2007
Publication Date: 5/19/2007
Citation: Waters, W.R., Palmer, M.V., Nonnecke, B.J., Thacker, T.C., Estes, M., Jacobs, W.R., Larsen, M.L. 2007. The Neonatal calf Tuberculosis Vaccine Model: Immune Responses to Protective and Non-protective Vaccines after Aerosol Challenge with Virulent Mycobacterium bovis [abstract]. The American Association of Immunologists. p. 43.51.

Interpretive Summary:

Technical Abstract: An attenuated Mycobacterium tuberculosis delta RD1 knockout and pantothenate auxotroph (mc**2 6030) vaccine failed to protect neonatal calves from a low dose, aerosol M. bovis challenge. In contrast, M. bovis bacille Calmette Guerin (BCG)-vaccinates had reduced tuberculosis-associated pathology as compared to non- and mc**2 6030-vaccinates. Mycobacterial colonization was not impacted by vaccination. Positive prognostic indicators associated with reduced pathology in the BCG-vaccinated group included: decreased antigen induced IFN-gamma, iNOS, IL-4, and MIP1-alpha responses and a diminished activation phenotype (i.e., decreased CD25+ and CD44+ cells and increased CD62L+ cells) in mycobacterial-stimulated mononuclear cell cultures. The calf sensitization and challenge model provides an informative screen for candidate tuberculosis vaccines before their evaluation in costly non-human, primates.

Last Modified: 8/24/2016
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