Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 5/19/2007
Publication Date: 5/19/2007
Citation: Waters, W.R., Palmer, M.V., Nonnecke, B.J., Thacker, T.C., Estes, M., Jacobs, W.R., Larsen, M.L. 2007. The Neonatal calf Tuberculosis Vaccine Model: Immune Responses to Protective and Non-protective Vaccines after Aerosol Challenge with Virulent Mycobacterium bovis [abstract]. The American Association of Immunologists. p. 43.51.
Technical Abstract: An attenuated Mycobacterium tuberculosis delta RD1 knockout and pantothenate auxotroph (mc**2 6030) vaccine failed to protect neonatal calves from a low dose, aerosol M. bovis challenge. In contrast, M. bovis bacille Calmette Guerin (BCG)-vaccinates had reduced tuberculosis-associated pathology as compared to non- and mc**2 6030-vaccinates. Mycobacterial colonization was not impacted by vaccination. Positive prognostic indicators associated with reduced pathology in the BCG-vaccinated group included: decreased antigen induced IFN-gamma, iNOS, IL-4, and MIP1-alpha responses and a diminished activation phenotype (i.e., decreased CD25+ and CD44+ cells and increased CD62L+ cells) in mycobacterial-stimulated mononuclear cell cultures. The calf sensitization and challenge model provides an informative screen for candidate tuberculosis vaccines before their evaluation in costly non-human, primates.