Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 5/16/2007
Publication Date: 5/16/2007
Citation: Bae, J., Suh, M., Hou, C.T., Kim, H. 2007. Lipase-induced production of 7, 10-dihydroxy-8(E)-octadecenoic acid from triolein by Pseudomonas aeruginosa PR3 [abstract]. American Oil Chemists' Society. Bio-1, #8.
Technical Abstract: Hydroxy fatty acids have gained attention because of their special properties such as higher viscosity and reactivity compared with other non-hydroxy fatty acids. The bacterial isolate Pseudomonas aeruginosa (PR3) had been reported to produce mono-, di-, and tri-hydroxy fatty acids from different fatty acids. Of those, 7,10-dihydroxy-8(E)-octadecenoic acid (DOD) was produced with high yield from oleic acid by PR3. Up to now, the substrates used for microbial HFA production were free fatty acids. However, triacylglycerides, specifically triolein, can be utilized as a substrate by enzyme systems involved in HFA production from oleic acid. In this study, we used triolein as a substrate and found that triolein could be efficiently utilized by PR3 to produce DOD. Triolein was first hydrolyzed into oleic acid by the triolein-induced lipase of P. aeruginosa and then the released oleic acid was converted to DOD by PR3. Results from this study demonstrated that natural vegetable oils, without being intentionally hydrolyzed, could be used as substrates for the production of value-added hydroxy fatty acids by PR3. In addition, we investigated the biological activities of DOD. DOD showed strong antibacterial activities against several food borne and plant pathogenic bacteria.