Submitted to: National Cotton Council Beltwide Cotton Conference
Publication Type: Abstract Only
Publication Acceptance Date: 1/9/2007
Publication Date: 6/25/2007
Citation: An, C., Saha, S., Jenkins, J.N., Scheffler, B.E., Ma, D-P., Stelly, D.M. 2007. MYB genes, a family of transcription fators: SNPS characterization, chromosomal location, and phylogenetic analysis [abstract]. National Cotton Council Beltwide Cotton Conference. p. 1620.
Technical Abstract: The R2R3-Myb transcription factors are involved in many plant physiological and biochemical processes including regulation of trichome length and density in Arabidopsis. In cotton, Gossypium spp.,the developmental regulation of some R2R3-Myb transcription factors are related to fiber differentiation and expansion. The objectives of this research were: single nucleotide polymorphism (SNP) characterization, chromosomal assignment, and phylogenetic analysis of six Myb genes in cotton. The putative designation of any tetraploid sequence to a locus or genome was based on the results of the phylogenetic tree and its relationship with the homologue sequence of the two diploid ancestral species. The SNaPshot MultiPlex Kit (Applied Biosystems, Foster City, CA) was used to detect SNP markers according to the manufacturer's protocol. There were 108 SNP (81 single base changes, 27 indels) identified from 8.3 kb of aligned sequences from three G. hirsutum lines, one G. barbadense, one G. tomentosum, one G. mustelinum, one G. herbaceum, and one G. raimondii line. There has been a different evolution pattern among genotypes in the A and D subgenomes of these lines with more SNP changes in the D subgenome than the A. Myb1 members were located to chromosome 13 and 18, Myb2 to 8, Myb3 could not be located to chromosome, Myb4 members to chromosomes 7 and 16, Myb5 to chromosome 11 and Myb6 members to 11 and 21.