|Holbrook, Carl - Corley|
Submitted to: Multicrop Aflatoxin and Fumonisin Elimination and Fungal Genomics Workshop-The Peanut Foundation
Publication Type: Abstract Only
Publication Acceptance Date: 12/15/2006
Publication Date: 2/1/2007
Citation: Cantonwine, E., Holbrook Jr, C.C., Wilson, D.M., Songsri, P., Pimratch, S., Guo, B. 2007. Genetic Response to Seed Colonizatin by Aspergillus flavus in Peanut. Proc. Aflatoxin Elimination Workshop p. 73.
Interpretive Summary: not required
Technical Abstract: Previous studies to evaluate peanut genotypes for in vitro resistance to seed colonization by Aspergillus flavus have not resulted in the development of cultivars with resistance to aflatoxin contamination in the field. New breeding lines showing pre-harvest field resistance to aflatoxin contamination were compared to field susceptible genotypes and genotypes showing in vitro resistance to colonization in an in vitro seed assay to determine whether seed colonization might be a resistance trait associated with the field resistant lines. Seed of each genotype were inoculated with spores of A. flavus and maintained at 25°C and 98% RH for 7 days. The percentage of seeds with sporulating colonies and severity was recorded. Due to contamination, data of only 10 genotypes are compared. The field resistant lines (511-CC and 522-CC) showed intermediate levels of percent incidence and severity, which were comparable to most of the susceptible lines. Based on this experiment, it does not appear that seed colonization by A. flavus is a significant trait affecting the field resistance to aflatoxin contamination by 511-CC or 522-CC.