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United States Department of Agriculture

Agricultural Research Service

Title: Map based cloning of cereal abiotic stress tolerance genes at the Australian Centre for Plant Functional Genomics)

item Collins, Nicholas
item Sutton, Timothy
item Schnurbusch, Thorsten
item Shi, Bu-jun
item Chen, Andrew
item Reinheimer, Jason
item Brule-babel, Anita
item Rivandi, Alireza
item Tester, Mark
item Gustafson, J
item Langridge, Peter

Submitted to: International Triticeae Mapping Initiative Workshop
Publication Type: Abstract Only
Publication Acceptance Date: 7/1/2006
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Map-based cloning is one of the approaches we are using to isolate cereal genes conferring tolerance to abiotic stresses. Targets include barley 4HL and wheat 7BL boron tolerance genes, a 1HL barley gene that influences shoot sodium accumulation, a 2HL barley frost tolerance gene, and a rye 7RS aluminium tolerance gene. New targets will be identified through drought and salinity germplasm characterization. Targeted marker generation using gene co-linearity with the small-genome grasses rice and Brachypodium is providing information about gene conservation and chromosomal inversions between the small-genome species and the triticeae. Various strategies are being used to map QTL traits as discrete loci. Trait loci are scored by monitoring element accumulation in shoots of hydroponically grown plants (Na and B), root length (B and Al), and frost-induced sterility using the AGRF (Adelaide) frost simulation chamber. Recovery and sequencing of target barley/wheat intervals is being facilitated by BAC libraries being made at ACPFG. Genomic libraries of transformation-competent large-insert (TAC) clones are being used to help facilitate functional testing by transformation. The wheat boron tolerance gene will be identified by mutant generation/sequencing. As a spin-off, the program is generating easy-to-use PCR-based markers useful for selection of the often difficult-to-score traits in breeding populations. Abiotic stresses in cereals are major agronomic problems for worldwide cereal production. The cloned genes will have a significant impact on the world's ability to improve cereal production on stress-related soils.

Last Modified: 05/26/2017
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