Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 12/8/2006
Publication Date: 12/8/2006
Citation: Kim, D., Bae, J., Kim, I., Kang, S., Hou, C.T., Kim, H. 2006. Production of biologically active hydroxy fatty acids from vegetable oils by Pseudomonas aeruginosa PR3 [abstract]. International Symposium on Biocatalysis and Bioenergy. J-4, p. 67.
Technical Abstract: Hydroxy fatty acids have gained industrial attention because of their special properties such as higher viscosity and reactivity compared with other non-hydroxy fatty acids. The bacterial isolate Pseudomonas aeruginosa (PR3) had been reported to produce mono-, di-, and tri-hydroxy fatty acids from different unsaturated fatty acids. Of those, 7,10-dihydroxy-8(E)-octadecenoic acid (DOD) was produced with high yield from oleic acid by PR3. Up to now, the substrates used for microbial HFA production were free fatty acids. However, triacylglycerides, specifically triolein can be utilized as a substrate by microbial enzyme systems involved in HFA production from oleic acid. In this study, we used triolein and some vegetable oils containing oleic acid as substrate for the production of hydroxy fatty acids by PR3. Triolein and vegetable oils could be efficiently utilized by PR3 to produce DOD. The starting compounds were first hydrolyzed into oleic acid by the substrate-induced lipase and then the released oleic acid was converted to DOD by PR3. Results from this study demonstrated that natural vegetable oils, without being intentionally hydrolyzed, could be used as an efficient substrate for the microbial production of value-added hydroxy fatty acids.