Submitted to: Journal of Microbiological Methods
Publication Type: Peer reviewed journal
Publication Acceptance Date: 8/1/2007
Publication Date: 10/1/2007
Citation: Akamatsu, H. O., Grünwald, N. J., Chilvers, M. I., Porter, L. D., and Peever, T. L. 2007. Development of codominant simple sequence repeat, single nucleotide polymorphism and sequence characterized amplified region markers for the pea root rot pathogen, Aphanomyces euteiches. Journal of Microbiological Methods 71: 82-86. Interpretive Summary: Aphanomyces root rot, caused by Aphanomyces euteiches, is the most damaging disease affecting peas worldwide. The present research identified several genetic markers that can be used to assess the genetic diversity within a population of A. euteiches. Identifying the genetic diversity in A. euteiches is important to pea breeders and plant pathologists. These markers can be used to select a genetically diverse group of individuals from the pathogen population to screen pea breeding lines for resistance to Aphanomyces root rot. This is necessary in order to develop pea varieties that provide durable resistance to a wide range of genetically diverse isolates of the pathogen in all growing regions of the world. These markers will also facilitate population genetic and evolutionary studies of this important plant pathogen.
Technical Abstract: Aphanomyces root rot, caused by Aphanomyces euteiches, is a major disease affecting peas worldwide. Here we report three kinds of codominant genetic markers for the pathogen including simple sequence repeats (SSRs), single nucleotide polymorphisms (SNPs) and sequence characterized amplified regions (SCARs). Of 69 primer sets tested, seven SSR, two SNP and two SCAR markers were codominantly polymorphic when scored by high resolution sieving agarose electrophoresis. These markers will facilitate population genetic and evolutionary studies of this important plant pathogen.