Submitted to: Springer Verlag
Publication Type: Book / chapter
Publication Acceptance Date: 8/1/2008
Publication Date: 1/17/2009
Citation: Li, S., Amstutz, P., Tang, C., Hang, J., Sandaram, A., Zhu, P., Shelton, D.R., Karns, J.S. 2009. DESIGN OF INTEGRATING WAVEGUIDE BIOSENSOR. Springer Verlag. p .389-401. Interpretive Summary: The Integrating Waveguide Biosensor is a new and novel instrument which can be used to detect a variety of pathogenic bacteria or toxins. The pathogenic agents are captured on the inner surface of glass capillary tubes (waveguides) using antibodies attached to the capillary wall. The pathogenic agents are then labeled with a second antibody which has a fluorescent dye attached. When the waveguide is illuminated with laser light, the fluorescent dyes emit light which is collected and channeled to the end of the waveguide where it is detected. The Biosensor has been optimized for the detection of the fluorescent dye Cy5, which allows for the detection of as few as 10 cells of Salmonella.
Technical Abstract: The Integrating Waveguide Biosensor allows for rapid and sensitive detection of pathogenic agents, cells and proteins via immunoassay or PCR products. The analytes are captured on the surface of the waveguide and then tagged with fluorescent labels. The waveguides are illuminated by excitation light at a 90 angle. The emitted light from fluorescent labels on the surface of the waveguide is efficiently collected and channeled to the detector at the end of the waveguide, while minimizing interference from the excitation light. Utilizing Cy5 as the fluorescent dye, a 635 nm diode laser for excitation and photomultiplier tube detector, the Integrating Waveguide Biosensor can detect approximately 10 captured cells of Salmonella.