Submitted to: Aquaculture Genome Technologies
Publication Type: Book / Chapter
Publication Acceptance Date: 9/27/2006
Publication Date: 8/1/2007
Citation: Rexroad III, C.E. 2007. Radiation Hybrid Mapping in Aquatic Species. Aquaculture Genome Technologies Chapter 18.
Technical Abstract: Understanding the mechanisms underlying the inheritance of traits has always been of great interest to humanity. The first documented studies in inheritance were conducted on peas in the mid 1800s by the Austrian monk Gregor Von Mendel. In the early 1900s, Thomas Morgan and colleagues experimented with the fruit fly Drosophila resulting in the construction of linear ordered maps that represented the inheritance of mutations through multiple generations. Since then, many advances in genetic mapping have been realized. One mapping strategy developed in the late 1960s, somatic cell hybrid genetics, became especially useful for mapping genes to chromosomes. In the 1990s further refinement of this technology included the use of radiation to break chromosomes into small fragments, resulting not only in the assignment of genes to chromosomes but in the construction of linear ordered maps of genes. Radiation hybrid maps have now been developed for humans, model organisms, and agriculturally important species. As the amount of publicly available genome tools and reagents increase for aquaculture species, radiation hybrid mapping strategies may be employed to integrate genetic maps, physical maps, and scaffolds of whole genome sequences.