Submitted to: CDFA Pierce's Disease Control Program Research Symposium
Publication Type: Proceedings
Publication Acceptance Date: 9/25/2006
Publication Date: 11/27/2006
Citation: Lin, H., Doddapaneni, H., Yao, J., Walker, A. 2006. Microarray gene expression analysis of grape plants in response to xylella fastidiosa infection, Pierce's Disease Control Program Research Symposium, San Diego, CA., November 27-29, 2006, p. 290-293. Interpretive Summary: Gene expression profiling using a custom high-density microarray chip of 20,020 grape gene probes showed significant variations in response among susceptible and resistant grape lines to infection with Xylella fastidiosa (Xf), the causal agent of Pierce’s Disease. Different gene expression patterns in response to Pierce’s Disease were identified and included genes associated with disease resistance, water stress, photosynthesis and cell wall synthesis. The results suggest that plant response to Xf infection are genotype and tissue dependent, and also vary over time. The gene expression results are available online at VitisExpDB, a relational database that houses annotated gene expression data (http://cropdisease.ars.usda.gov/~fruit_tree/). The database provides a resource to the grape community for functional gene analysis of both vinifera and non-vinifera grape varieties.
Technical Abstract: Transcriptional profiling using a custom high-density microarray chip of 20,020 Vitis transcripts showed significant variations in responses between the susceptible and resistant genotypes to Xylella fastidiosa (Xf) infection. Differentially expressed transcripts reflecting spatial and temporal responses to PD involved in metabolic processes such as diseases resistance, water stress, photosynthesis and cell wall synthesis were identified. The results suggest that Vitis responses to Xf are genotype and tissue dependent, and are stage specific. VitisExpDB is an online MySQL-php driven relational database that houses annotated EST data (http://cropdisease.ars.usda.gov/~fruit_tree/). The database will provide genomic resource to grape community for functional analysis for both vinifera and non-vinifera grape varieties and aid in annotation of the grape genome.