|Holbrook, Carl - Corley|
Submitted to: Aflatoxin Elimination Workshop Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 9/29/2006
Publication Date: 10/13/2006
Citation: Guo, B., Chen, H., Dang, P.M., Luo, M., Liang, X., He, G., Holbrook, C.C., Kvien, C.K. 2006. Development of peanut EST (expressed sequence tag)-based genomic resources and tools [abstract]. In: Proceedings of the Annual Aflatoxin Elimination Workshop, October 16-18, 2006, Fort Worth, Texas.
Technical Abstract: U.S. Peanut Genome Initiative (PGI) has widely recognized the need for peanut genome tools and resources development for mitigating peanut allergens and food safety. Genomics such as Expressed Sequence Tag (EST), microarray technologies, and whole genome sequencing provides robotic tools for profiling genes. In spite of continuous decrease in DNA sequencing costs, it is improbable that many large plant genomes, such as peanut, will be sequenced in the near future. However, partially sequencing of large numbers of expressed genes (ESTs) can deliver substantial amounts of genetic information that will allow comparative and functional studies. The target of PGI is to have at least 150,000 ESTs by 2008. Notable research progress has been made recently in development of peanut ESTs. Up to today, total 43,296 cDNA clones from ten peanut cDNA libraries have been sequenced. After comparison and assembly of overlapping sequences, about 10,000 unique sequences have been identified. These sequence data will be available to the community in order to develop genomic tools and resources for deciphering the chromosomal location and biological function of genes in the peanut genome and mitigating peanut food safety issues. A proposal of peanut 70-mer oligo microarray consisting of more than 10,000 gene-elements is under discussion in collaboration with TIGR (the Institute for Genomic Research). The peanut oligo array will be available by 2008 for peanut international community.