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Title: FED-BATCH AND CONTINUOUS PRODUCTION OF MANNITOL BY A HETEROFERMENTATIVE LACTIC ACID BACTERIUM

Author
item RACINE, F - ZUCHEM
item Saha, Badal

Submitted to: UJNR Food & Agricultural Panel Proceedings
Publication Type: Proceedings
Publication Acceptance Date: 10/27/2006
Publication Date: 10/27/2006
Citation: Racine, F.M., Saha, B.C. 2006. Fed-batch and continuous production of mannitol by a heterofermentative lactic acid bacterium. In: McKeon, T., Barton II, F., editors. Proceedings of the United States-Japan Cooperative Program in Natural Resources Food and Agricultural Panel 35th Annual Meeting, October 21-28, 2006, Rohnert Park, Sonoma, California. p. BT13-BT17.

Interpretive Summary:

Technical Abstract: Improved fermentation processes were developed for the production of mannitol by a heterofermentative lactic acid bacterium (Lactobacillus intermedius NRRL B-3693). A fed-batch protocol overcame limitations caused by high substrate concentrations. The process was developed using primarily corn steep liquor as an inexpensive industrial nutrient source. The fed-batch process resulted in the accumulation of 176 g mannitol from 184 g fructose and 92 g glucose per L of final fermentation broth in 30 h with a volumetric productivity of 5.9 g/L-h. Further increases in volumetric productivity of mannitol were obtained in a continuous cell-recycle fermentation process that reached more than 40 g/L-h. This is the first report of such a high volumetric productivity of mannitol by a heterofermentative lactic acid bacterium.