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Title: Oxygenases for aliphatic hydrocarbons and fatty acids

item Hou, Ching

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 12/8/2006
Publication Date: 12/8/2006
Citation: Hou, C.T. 2006. Oxygenases for aliphatic hydrocarbons and fatty acids [abstract]. International Symposium on Biocatalysis and Bioenergy. G-4.

Interpretive Summary:

Technical Abstract: Oxygenases catalyzing the insertion of oxygen into either aliphatic hydrocarbons or fatty acids have great similarity. There are two classes of oxygenases: monooxygenases and dioxygenases. Dioxygenase inserts both atoms of molecular oxygen into a substrate, whereas monooxygenase incorporates one atom of molecular oxygen into a substrate while the other is reduced to water. These enzymes are sometimes referred to as mixed function oxygenases or mixed function oxidases since they are apparently bifunctional, carrying out oxidase activity on one site and oxygenase activity on the other. Many oxygenated fatty acids were produced by monooxygenase reactions, for examples: oleic acid to 7,10-dihydroxy octadecenoic acid by Pseudomonas aerugenosa PR3, and many n-3 and n-6 unsaturated fatty acids to oxygenated unsaturated fatty acid products by Bacillus megaterium ALA2. There are three types of monooxygenases: cytochrome P450, diiron-cluster, and catalytically self sufficient cytochrome P450 monooxygenases. In most cases, monooxygenases for aliphatic hydrocarbons or fatty acids catalyze both hydroxylation and epoxidation reactions. After analyzing the reaction products and comparing them with the monooxygenase system in known strains, we concluded that the monooxygenase system in strain ALA2 is possibly a catalytically self-sufficient cytochrome P450 monooxygenase.