Submitted to: American Phytopathological Society Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 1/1/2007
Publication Date: 7/31/2007
Citation: Guerra-Moreno, A.S., Keremane, M.L., Brlansky, R.H., Lee, R.F. 2007. The Complete Sequence of the Cytoplasmic Citrus Leprosis Virus, (CCLV) and its Genome Organization. Phytopathology 97: S 174 Interpretive Summary:
Technical Abstract: Leprosis, an emerging invasive disease of citrus in Central America, has been present in South America for several years. Leprosis does not occur in the U.S. or the Caribbean Islands, but these regions are threatened by the disease. We have described the association of a bipartite, positive-sense RNA virus associated with the cytoplasmic citrus leprosis. The CCLV RNAs were identified from a cDNA library created from citrus naturally infected with the cytoplasmic leprosis disease from Panama. Here we report the complete sequence of the CCLV bipartite genome. RNA 1 possesses two open reading frames (ORFs). ORF 1 in RNA 1 encodes a putative 276 kDa polyprotein containing domains similar to the Sindbis-like virus super group having putative methyltransferase, cysteine protease, RNA helicase and RNA dependent RNA polymerase motifs. ORF 2 in RNA 1 shows no similarity with other sequences in the Genbank. RNA 2 has three ORFs. While ORFs 1 and 3 of RNA 2 show no similarity with sequences in the Genbank, ORF 2 encodes a putative 30.6 kDa viral movement protein.