Submitted to: Symposium on Avian Pneumovirus
Publication Type: Proceedings
Publication Acceptance Date: 9/8/2006
Publication Date: 9/27/2006
Citation: Yu, Q., Estevez, C., Kapczynski, D.R. 2006. Characterization of monoclonal antibodies produced against Avian metapneumovirus Sybtype C. Symposium on Avian Pneumovirus.
Technical Abstract: Monoclonal antibodies (MAbs) were prepared against avian metapneumovirus (aMPV) subtype C (aMPV/Minnesota/turkey/1a/97). Six MAbs were selected based on ELISA activities and characterized by isotyping, neutralization test, Western blot analysis, and immunohistochemistry (IHC) assay. The results showed that three MAbs (3E, 9D and 12C) belonged to IgG1 subclass, whereas the other three (5D, 8E and 16E) were of the IgG2a subclass. All six MAbs did not neutralize aMPV infectivity at a detectable level, but reacted with both denatured and non-denatured forms of the nucleocapsid (N) protein of aMPV, suggesting that these MAbs may recognize structurally independent epitopes of the N protein. These MAbs provide new tools and methods for investigating aMPV infection, pathogenesis, and diagnosis of aMPV disease.