Submitted to: Latex International Conference Proceedings
Publication Type: Proceedings
Publication Acceptance Date: 3/1/2006
Publication Date: 7/25/2006
Citation: Cornish, K., McMahan, C.M., Xie, W., Williams, J., Nguyen, K.C., Kostyal, D., Marsh, D.T. 2006. Astm d1076 category 4 latex and quantifying guayule (nrg) and hevea (nr) latex protein. Proceedings of the International Latex Conference. pp. 246-261. Interpretive Summary: Natural rubber is a strategic raw material used in enormous amounts in over 40,000 applications. The United States, with no natural rubber domestic production of its own, is completely dependent upon imports. Our research supports the commercial development of guayule as a source of hypoallergenic latex, especially for the medical products market, and the development of annual rubber-producing crops.
Technical Abstract: Guayule latex is commercially available as a low protein natural rubber latex (Yulex®) which does not contain any protein that can be detected by the ASTM D6499 antigenic protein standard developed to quantify Hevea natural rubber latex (NRL) antigenic protein. In this paper, we discuss how best to quantify the proteins in guayule latex and Hevea latex using a minor modification of the modified Lowry procedure described in ASTM D5712 and compare this to quantification of proteins extracted from guayule and Hevea latex films. We also address the development of guayule-specific immunochemical methods to determine latex purity and to accurately quantify very small amounts of extractable protein from products.