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ARS Home » Midwest Area » Peoria, Illinois » National Center for Agricultural Utilization Research » Mycotoxin Prevention and Applied Microbiology Research » Research » Publications at this Location » Publication #198139
Title: COMPLETE GENETIC LINKAGE MAPS FROM AN INTERSPECIFIC CROSS BETWEEN FUSARIUM CIRCINATUM AND FUSARIUM SUBGLUTINANS

Author
item DE VOS, L - UNIVERSITY OF PRETORIA
item MYBURG, A. - UNIVERSITY OF PRETORIA
item WINGFIELD, M - UNIVERSITY OF PRETORIA
item Desjardins, Anne
item GORDON, T - UNIVERSITY OF CALIFORNIA
item WINGFIELD, B - UNIVERSITY OF PRETORIA

Submitted to: Fungal Genetics and Biology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 2/17/2007
Publication Date: 2/28/2007
Citation: De Vos, L., Myburg, A.A., Wingfield, M.J., Desjardins, A.E., Gordon, T.R., Wingfield, B.D. 2007. Complete genetic linkage maps from an interspecific cross between Fusarium circinatum and Fusarium subglutinans. Fungal Genetics and Biology. 44(8):701-714.

Interpretive Summary: In our laboratory we developed a large number of Fusarium strains and supplied them to scientific collaborators in South Africa. DNA marker analysis of these strains showed the unexpected close genetic relationship between two Fusarium species, one that causes maize ear rot, and another that causes pitch canker of pine trees. These DNA markers should provide new tools to pathologists and breeders who are trying to control the fungi that cause maize ear rot and pine pitch canker, both of which are economically important diseases in the United States.

Technical Abstract: Fusarium isolates associated with the Gibberella fujikuroi species complex include many important fungal pathogens of agricultural crops and trees. In this study an interspecific hybrid between F. circinatum and F subglutinans was used to compile a genetic linkage map. A framework map was constructed using a total of 578 AFLP markers together with the mating type (MAT-1 and MAT-2) genes and the histone (H3) gene. Twelve major linkage groups were identified (n=12) in Fusarium. Fifty percent of the markers showed significant deviation from the expect 1:1 transmission ratio in a haploid F1 cross (P<0.05). The transmission of the markers on the linkage map was biased towards alleles of the F. subglutinans parent, with an estimated 60% of the genome of F1 individuals contributed by this parent. This map will serve as a powerful tool to study the genetic architecture of interspecific differentiation and pathogenicity in the two parent genomes.