|Mcgrath, J Mitchell - Mitch|
Submitted to: Annual Beet Sugar Development Foundation Research Report
Publication Type: Research Notes
Publication Acceptance Date: 5/16/2006
Publication Date: 7/1/2006
Citation: Pan, D., McGrath, J.M. 2006. Existence of resistance gene analogues in sugar beet and related germplasm. 2005 Annual Beet Sugar Development Foundation Research Report. p. D40-D46.
Technical Abstract: Host-plant disease resistance is a useful characteristic in sugar beet breeding. Plant R (resistance) genes confer resistance to a variety of pathogens through a yet unclear mechanism. Conserved R gene motifs had been exploited previously to identify 47 resistance gene analogues (RGAs) in sugar beet, for which primers had been designed and reported. Using a PCR approach, these RGAs were tested for presence in genomic DNA of sugar beet and related germplasm and in a range of cDNA libraries representing various tissue types and environmental conditions. All of the studied RGAs were found to be present in the genomic DNA of at least two of the studied germplasms and expressed in at least two of the studied cDNA libraries. This exploratory experiment confirms the usefulness of the set of sugar beet specific RGA primers in studying other varieties of sugar beet and cultivars of Beta vulgaris. Variation noted may constitute useful genetic variation in breeding programs.