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Title: QUANTITATIVE TRAIT LOCI FOR WINTER HARDINESS COMPONENT TRAITS IN OAT

Author
item WOOTEN, DAVID - NORTH CAROLINA STATE UNIV
item Livingston, David
item LYERLY, JEANETTE - NORTH CAROLINA STATE UNIV
item MURPHY, J - NORTH CAROLINA STATE UNIV

Submitted to: American Oat Workers Conference Proceedings
Publication Type: Other
Publication Acceptance Date: 6/15/2006
Publication Date: 7/24/2006
Citation: Wooten, D.R., Livingston, D.P., Lyerly, J.H., Murphy, J.P. 2006. Quantitative trait loci for winter hardiness component traits in oat. American Oat Workers Conference Proceedings. p. 63.

Interpretive Summary: Winter hardiness is an important but difficult trait for breeders to improve. The discovery of pieces of DNA that are associated with Winterhardiness and can be readily identified may improve the chances of breeders to find more winter hardy types of oats. Two winter hardy oat lines were crossed and their offspring were evaluated for several characteristics related to winter hardiness. Several DNA pieces were identified that could help breeders identify more winter hardy lines without extensive freeze testing in the field or in the laboratory.

Technical Abstract: Winter hardiness is an important limitation to winter oat (Avena byzantina and A. sativa) production in much of North America, but field evaluation of winter hardiness is difficult. The discovery of quantitative trait loci for winter hardiness should allow markers assisted selection for winter hardiness. A population of 135 recombinant inbred lines (RIL) derived from a cross between winter hardy ‘Norline’ and winter tender ‘Fulghum’ were evaluated for the winter hardiness component traits field survival, freeze tolerance, maturity, vernalization and photoperiod response. The RIL were screened with RFLP markers in regions of suspected QTL. QTL were identified with single factor analysis and multiple interval mapping using QTL cartographer. QLT for field survival, freeze tolerance, and vernalization were found on chromosomes 17 and 7C. Breeders could utilize these QTL to improve oat winter hardiness through marker assisted selection.