Submitted to: American Journal of Potato Research
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 3/6/2008
Publication Date: 10/1/2008
Citation: Hale, A.L., Reddivari, L., Bamberg, J.B., Miller, J.C. 2008. Interspecific variability for antioxidant activity and phenolic content among solanum species. American Journal of Potato Research. 85(5):332-341. Interpretive Summary: Potato is the most important US vegetable. Its high per capita consumption makes it a good food to deliver beneficial nutrients to population's diet. Colored vegetables have received much attention as being high in antioxidants, nutrients which have multiple health benefits, but potato antioxidant levels can also be high. When we examined the limits of antioxidants in exotic potato relatives, some had five times as much as the highest cultivated potato. If these high antioxidant levels from exotic species can be bred into new potato varieties, it could have a significant positive impact on human health.
Technical Abstract: Total antioxidant activity was evaluated in 47 tuber-bearing Solanum species. Accessions identified in a broad screen as having high antioxidant activity were fine screened via HPLC to determine specific phenolic compounds present. The objective of the study was to identify tuber-bearing Solanum species that are higher in antioxidants than currently available cultivated varieties and breeding lines. These accessions will be used as a source of germplasm to develop potato cultivars containing increased levels of antioxidant compounds. In a broad screen for total antioxidant activity, the 47 related, tuber-bearing species showed a wider range of variability than cultivated varieties and breeding lines. Solanum pinnatisectum and S. jamesii accessions consistently ranked among the highest in antioxidant activity and phenolic content. Based on the DPPH assay, antioxidant activity in the wild species ranged from 42 to 892 µg trolox eq gfw-1. HPLC analysis revealed that the phenolic content of the species was primarily composed of chlorogenic and caffeic acids. Other phenolics identified were p-coumaric acid, rutin hydrate, vanillic acid, epicatechin, t-cinnamic acid, gallic acid, and salicylic acid. The highest phenolic content found among the accessions was five-fold higher than the highest of the cultivated genotypes.