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item Farnham, Mark

Submitted to: Germplasm Release
Publication Type: Germplasm Release
Publication Acceptance Date: 7/25/2006
Publication Date: 7/25/2006
Citation: Farnham, M.W. Notice of resease of downy mildew resistant green sprouting broccoli inbred line usvl089. USDA, Agricultural Research Service, Washington, D.C. July 25, 2006.

Interpretive Summary:

Technical Abstract: Downy mildew is one of the most destructive diseases of broccoli and other Brassica oleracea L. crops. This fungal disease, caused by the biotrophic parasite Peronospora parasitica (Pers. Fr.), is a problem worldwide. Fungicide application can provide some control of downy mildew in broccoli. However, the use of resistant broccoli cultivars is an alternative control method that could provide a practical, long-term, and environmentally-benign means to limit crop damage due to this disease. The USDA-ARS broccoli breeding program at the U.S. Vegetable Laboratory in Charleston, South Carolina, developed an inbred line of green-sprouting broccoli that is a uniform and true-breeding source of resistance to downy mildew. The new inbred designated USVL089 is a doubled haploid line derived from anther cultures of the heterozygous F1 hybrid Everest. Unlike Everest, USVL089 exhibits a high level of homozygosity. It is self-incompatible and must be self-pollinated to produce seed. USVL089 exhibits a high level of downy mildew resistance at the cotyledon stage, the 3- to 4-true leaf stage, and throughout growth to vegetable maturity. The resistance is conditioned by a single dominant gene. Hybrids made using USVL089 as a parent express the same high level of resistance. USVL089 is an excellent source for a downy mildew resistance gene that can readily be transferred to new breeding populations; it should prove useful to broccoli and other cole crop breeders focused on developing disease resistant hybrids.