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United States Department of Agriculture

Agricultural Research Service


item Guber, Andrey
item Karns, Jeffrey
item Pachepsky, Yakov
item Sadeghi, Ali
item Van Kessel, Jo Ann
item Dao, Thanh

Submitted to: ASA-CSSA-SSSA Annual Meeting Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 5/8/2006
Publication Date: 11/12/2006
Citation: Guber, A.A., Karns, J.S., Pachepsky, Y.A., Sadeghi, A.M., Van Kessel, J.S., Dao, T.H. 2006. Differences in release and transport of manure-borne E. coli and Enterococci in grass buffer conditions. ASA-CSSA-SSSA International Meeting, November 12-16, 2006, Indianapolis, Indiana. CD-ROM.

Interpretive Summary:

Technical Abstract: Echerichia coli and Enterococci are common indicator organisms used to detect potential fecal contamination of surface and ground water and to determine water quality. Coupled monitoring of both organisms in karst demonstrated differences in dynamics of their concentrations in waters that were reached by fecal contamination. Little if anything is known about the differences in transport of the two indicator organisms in soil, or about the differences in their overland transport. The objective of this work was to test the hypothesis that the two organisms have similar patterns of release from animal feces and subsequent transport in the environment. The turfgrass soil sod was placed into boxes that were 10 cm deep, 30 cm wide and 200 cm long, and had top section separated to monitor the release and infiltration of bacteria that occurred in the rest of the box. Boxes with either live or dead grass were placed under rainfall simulator and tilted to have 4 % slopes. Dairy manure was broadcast on the upper 30-cm section. Rainfall was simulated for 90 min and runoff samples were collected and analyzed for Br, E. coli, Enterococci and turbidity. Significant differences in release kinetics of E. coli and Enterococci were found. Differences in release but not in the subsequent transport were observed for E. coli and Enterococci. The change from the first order release kinetics to the zero order release kinetics after 1 h of rainfall was observed. Because both E. coli and Enterococci are currently used as indicator organisms for manure-borne pathogens, differences in their release rates may affect the efficiency of using these organisms as indicators of fecal contamination from lands in agricultural use.

Last Modified: 10/19/2017
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