Submitted to: Biomed Central (BMC) Genomics
Publication Type: Peer reviewed journal
Publication Acceptance Date: 1/16/2006
Publication Date: 1/16/2006
Citation: Lobo, N.F., Behura, S.K., Aggarwal, R., Chen, M., Collins, F.H., Stuart, J.J. 2006. Genomic analysis of a 1 mb region near the telomere of hessian fly chromosome x2 and avirulence gene vh13. Biomed Central (BMC) Genomics 7:7. Interpretive Summary: The Hessian fly [Mayetiola destructor (Say)] is one of the most destructive pests of wheat. Host plant resistance is the most effective and cost-efficient way to control the damage caused by this pest. The challenge for the host plant resistance strategy is that resistance conferred by specific genes are short lived, effective for only 6 to 8 years. To develop more durable resistance cultivars, we need to understand how the insect can overcome host plant resistance so quickly. This research is toward to isolate an avirulence gene that will help to understand how the Hessian fly overcomes host plant resistance.
Technical Abstract: Chromosome walking and FISH were utilized to identify a contig of 50 BAC clones near the telomere of the short arm of Hessian fly chromosome X2 and near the avirulence gene vH13. These clones enabled us to correlate physical and genetic distance in this region of the Hessian fly genome. Sequence data from these BAC ends encompassing a 760 kb region, and a fully sequenced and assembled 42.6 kb BAC clone, was utilized to perform a comparative genomic study. In silico gene prediction combined with BLAST analyses was used to determine putative orthology to the sequenced dipteran genomes of the fruit fly, Drosophila melanogaster, and the malaria mosquito, Anopheles gambiae, and to infer evolutionary relationships.