Submitted to: Journal of American Society for Mass Spectrometry
Publication Type: Abstract Only
Publication Acceptance Date: 6/13/2006
Publication Date: 8/1/2006
Citation: Thannhauser, T.W., Yang, Y., Zhang, S., Sherwood, R., Isaac, I. 2006. High throughput protein identification using 2-dimensional difference gel electrophoresis and robotic spot picking for aluminum tolerance-related maize root tip proteins. Journal of American Society for Mass Spectrometry. 17:46.
Technical Abstract: Two-dimensional difference gel electrophoresis (2-D DIGE) is the most effective method utilized to carry out gel-based quantitative proteomics. Unfortunately, the most popular image analysis software used to process DIGE images (DeCyder) produces picking coordinates in a format that is incompatible with most commercially available spot picking robots. To achieve the full promise of proteomic research it is necessary to perform protein identification experiments on such a scale that automation is required. Thus, there is a need for transformation software to convert the DeCyder generated picking coordinates to a format compatible with commonly available robots. Here we present a protocol using 2-D DIGE, DeCyder and the ProPic spot picking robot incorporating such a program that greatly facilitates protein identification.