Submitted to: Phytochemical Society of North America Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 7/12/2006
Publication Date: 7/12/2006
Citation: Berhow, M.A., Duval, S.M. 2006. Extraction and characterization of anthocyanins from black seed hulls [abstract]. Phytochemical Society of North America Proceedings. P. 22. Available: www.psna-online.org/PSNAabst06.pof.
Technical Abstract: New methodology has been developed for the preparative scale extraction and purification of anthocyanins from "black" seed coats and hulls. A combination of physical fractionation, extraction, flash chromatography and preparative chromatography has resulted in the isolation of gram quantities of purified anthocyanins from black soybeans, black beans and cuphea. The anthocyanin structure was determined by LC-MS. The methodology developed can be used to quantitate the levels of the anthocyanins in various cultivars and growing conditions. The quantities being isolated allow for the evaluation of their insecticidal, fungicidal and herbicidal activities in a variety of bioassay systems.