Submitted to: Journal of Phytopathology
Publication Type: Abstract Only
Publication Acceptance Date: 5/1/2006
Publication Date: 6/1/2006
Citation: Paul, C., Strutz, D., Hartman, G.L. 2006. Molecular markers linked to soybean sclerotinia stem rot resistance using bulk segregant analysis [abstract]. Journal of Phytopathology. 96:S91.
Technical Abstract: Sclerotinia stem rot is a common disease of soybean in the northern growing regions of the U.S. One method to manage this disease is host resistance. The objective of this experiment was to determine if bulk segregant analysis could be used to develop markers associated with genes or genomic regions for resistance. Plants of F4:5 derived lines of PI194634 x Merit were scored for resistance 2 weeks after inoculation using the cut stem method. One disease assessment used measurements made of stem lesion lengths and the other used a visual 0 (most resistant) to 9 (most susceptible) scale. Two bulk samples of DNA were extracted based on those lines that were identified as resistant and susceptible, respectively, using the two assessment methods. The bulk samples were screened for differences using fluorescently labeled SSR primers to identify polymorphic markers associated with genes for resistance to Sclerotinia stem rot. Using bulked segregant analysis, polymorphic markers were identified and a number of markers closely linked to a specific region of the genome were identified based on the disease assessment. Linkage analysis was performed to estimate the genetic distance of the linked markers. Significant markers tightly linked to the gene may prove to be instrumental in positional cloning and hasten the development of soybean lines with resistance to Sclerotinia stem rot.