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ARS Home » Midwest Area » Urbana, Illinois » Soybean/maize Germplasm, Pathology, and Genetics Research » Research » Publications at this Location » Publication #194967


item Bandyopadhyay, Ranajit
item Paul, Chandra
item Twizeyimana, Mathias
item Adeleke, Remi
item Miles, Monte
item Hartman, Glen

Submitted to: Journal of Phytopathology
Publication Type: Abstract Only
Publication Acceptance Date: 5/1/2006
Publication Date: 6/1/2006
Citation: Bandyopadhyay, R., Paul, C., Twizeyimana, M., Adeleke, R., Miles, M.R., Hartman, G.L. 2006. Identification and development of resistance to soybean rust in nigeria [abstract]. Journal of Phytopathology. 96:S8.

Interpretive Summary:

Technical Abstract: Soybean rust is now endemic in Nigeria after it was first reported in 1999. The objectives of our research were to identify sources of resistance and polymorphic markers between combinations of resistant and susceptible lines. To identify sources of resistance, field, screen house and laboratory environments were used. In the field, three test rows in between infector rows provided uniform disease pressure. In the screen house, seedlings inoculated with urediniospores suspensions were assessed. In the laboratory, pieces of detached leaves placed on 1% agar amended with 10 ppm kinetin were used to evaluate resistance. More than 150 lines from the IITA soybean breeding program and nearly 100 lines from U.S. soybean germplasm collection were evaluated. One line from the U.S. germplasm collection was most resistant showing only hypersensitive flecks without any sporulation. Another U.S. line and a breeding line from Uganda had red brown resistant lesions. Several of these lines are being used in a crossing program to breed for rust resistance in Nigeria. To identify polymorphic markers between combinations of resistant and susceptible lines, 20 identified resistant lines and four susceptible lines were genotyped using SSR markers. The molecular marker data is being used to identify parents to create mapping populations to study QTLs for rust resistance.