Submitted to: Theoretical and Applied Genetics
Publication Type: Peer reviewed journal
Publication Acceptance Date: 8/2/2006
Publication Date: 10/24/2006
Citation: Zhao, H., Liu, X., Chen, M. 2006. H22, a major resistance gene to the hessian fly (mayetiola destructor), is mapped to the distal region of wheat chromosome 1ds. Theoretical and Applied Genetics. Interpretive Summary: The Hessian fly [Mayetiola destructor (Say)] is one of the most destructive pests of wheat. Host plant resistance is the most effective and cost-efficient way to control the damage caused by this pest. The challenge for the host plant resistance strategy is that resistance conferred by single genes is short lived, effective for only 6 to 8 years. More durable resistance could be achieved by breeding wheat cultivars with multiple resistance genes in a single cultivar, so called gene pyramiding. To achieve gene pyramiding of multiple genes, we need markers that are tightly linked with individual resistance genes. The current research identified several markers that are tightly linked with the resistance gene H22, one of the major genes providing resistance to the Hessian fly. These markers will accelerate the use of H22 and gene pyramiding to bring more durable cultivars for Hessian fly resistance to the fields.
Technical Abstract: H22 is a major resistance gene that confers a high level of antibiosis to the larvae of the Hessian fly [Mayetiola destructor (Say)]. The H22 gene was previously assigned to wheat chromosome 1D through monosomic analysis (Raupp et al., 1993). The objective of this study was to identify molecular markers that are tightly linked to this gene for marker-assisted selection for wheat breeding, and to further map this gene toward map-based cloning. Forty-five 1D-specific Simple Sequence Repeat (SSR) and Sequence-Tagged Site (STS) markers were evaluated for linkage to H22 using a segregating population consisting of 192 F2:3 families, which were derived from the cross Tugela-Dn1×KS85WGRC01(H22). The STS Xhor2kv and SSR Xgdm33 are two flanking markers that are tightly linked to H22 at genetic distances of 0.3 and 1.0 cM, respectively. Five other SSR markers including Xgpw7082, Xwmc147, Xcfd15, Xwmc432 and Xwmc336 were also linked to H22 at the distance from 0.8 to 20.8 cM. Analysis using Chinese Spring (CS) wheat deletion lines revealed that all these H22-linked markers are located distal to the breakpoint of del 1DS-5, indicating that the H22 gene is located at the distal 30% region on the short arm of wheat chromosome 1D.