|Maroon Lango, Clarissa|
|Hsu, Hei Ti|
Submitted to: Phytopathology
Publication Type: Abstract Only
Publication Acceptance Date: 3/30/2006
Publication Date: 6/1/2006
Citation: Maroon-Lango, C.J., Aebig, J., Hammond, J., Hsu, H.T. 2006. Molecular and biological characterization of a novel ilarvirus in bacopa. Phytopathology. 96:S73.
Technical Abstract: Bacopa plants (Sutera cordata cv. Snowflake) with virus-like symptoms including chlorosis and leaf dimpling were obtained from nurseries in Maryland. A virus was mechanically transmitted to Chenopodium quinoa, Cucumis sativus, Hyoscyamus niger, Lycopersicon esculentum (tomato ‘Roma’), Nicotiana glutinosa,and N. tabacum, but not to Cucurbita maxima, Glycine max, L. esculentum (‘Rutgers’), N. benthamiana, N. edwardsonii, N. sylvestris, Spinacea oleracea and Zinnia angustifolia. In N. tabacum, faint chlorotic spots developed 2-3 days after inoculation, later developing into necrotic spots, lines, and rings. Symptoms were more apparent in plants grown at between 60 and 72ºF, but would disappear above 75ºF. No serological reactions were observed with antiserum to Broadbean wilt virus (previously reported from bacopa) or Tobacco streak virus. Partial purification of the virus resulted in a banding profile in sucrose gradients typical of multicomponent viruses. RT-PCR using the Ilarvirus Group primers (Agdia, Inc., Elkhart, IN.) yielded a ca. 450-bp product which shared 80-87% nucleic acid and 83% amino acid identities with Parietaria mottle ilarvirus. Cloning and sequencing of almost the entire RNA1, more than the 5’ half of RNA 2, and the 5’ half of RNA 3 of the bacopa virus revealed that it is a distinct ilarvirus, for which we propose the name Bacopa chlorosis virus.