Submitted to: Maize Genetics Conference Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 1/20/2006
Publication Date: 3/9/2006
Citation: Okagaki, R., Jacobs, M., Schneeman, M., Kynast, R., Lawrence, C.J., Kamps, T., Rines, H.W., Weber, D., Bass, H.W., Phillips, R. 2006. A comparison of centromere mapping techniques [abstract]. 48th Annual Maize Genetics Conference Program and Abstracts. p. 68. Interpretive Summary:
Technical Abstract: We have mapped maize centromeres using telosomes and isochromosomes produced by spontaneous chromosome breaks, radiation-induced chromosome breaks recovered in T-B translocation lines or in oat-maize radiation hybrid lines, single-locus FISH, and half-tetrad analysis. These data have been compiled together with results from other work including Ed Coe's retrospective analysis of genetic data, Bor-Yaw Lin's work with T-B translocation lines and r-X1 induced terminal deficiencies, and Laurie Anderson's recombination nodule map. The data were insufficient to make useful comparisons for chromosomes 5, 7, and 8. Centromere locations on chromosomes 2, 4, 6, 9, and 10 were consistent with all, or all but one, of the studies. Results clustered in a distinct region spanning less than 5% of the genetic map. In contrast, centromere positions on chromosomes 1 and 3 were scattered over 10% or more of the genetic map. The inconsistencies found for centromere map positions on 1 and 3 were not obviously linked to a particular mapping technique, nor were they tied to materials used. Indeed, the same material used in different studies gave different centromere locations on chromosome 1.