Submitted to: Plant and Animal Genome VX Conference Abstracts
Publication Type: Abstract only
Publication Acceptance Date: 10/5/2005
Publication Date: 1/14/2006
Citation: Figueroa, D.M., Amarillo, I.E., Ring, B.C., Strobel, C.E., Lawrence, C.J., Bass, H.W. 2006. A cytogenetic map of maize in oat addition lines using sorghum BACs as FISH probes [abstract]. Plant and Animal Genome XIV: Final Abstract Guide. p. 367. Interpretive Summary:
Technical Abstract: We are developing a pachytene cytogenetic FISH map of the maize genome using sorghum BACs corresponding to the 90 maize Core Bin Marker (CBM) loci. These loci were chosen because they are uniformly distributed and they delineate the genetic bins derived from the UMC98 (Genetic 2005) maize linkage maps. We are using the single-locus cytogenetic FISH mapping system previously described by Koumbaris and Bass (2003, Plant J. 35:647). Maize marker-selected sorghum BACs are used as FISH probes on maize pachytene chromosome spreads from alien addition lines of oat. In addition, we have initiated an RFLP full-length insert sequencing (FLIS) project to enable in silico screening for suitable sorghum BACs. The protocol for selecting sorghum BACs is illustrated using the RFLP probe umc161 (CBM 1.11, GenBank Acc. AY771212). Progress towards detailed mapping of maize 9 with emphasis on centromeric loci will also be presented. The completion of a cytogenetic map will provide a means for integrating the physical, genetic, and cytological maps of maize with an added dimension of genome-wide cross-anchoring to the maps of sorghum. The project is described at cytomaize.org and mapping and image data are released through MaizeGDB. This project will provide insights into the structure of the maize genome, while creating new technologies and reagents for chromosome research.