Submitted to: Proceedings Sunflower Research Workshop
Publication Type: Proceedings
Publication Acceptance Date: 3/17/2006
Publication Date: 3/17/2006
Citation: Feng, J., Vick, B.A., Jan, C.C. Screening of sunflower BAC library for the identification of specific BAC clones. 28th Sunflower Research Workshop, January 11-12, 2006, Fargo, ND. Available: http://www.sunflowernsa.com/research/research-workshop/documents/Feng_BAC_06.pdf Interpretive Summary: Genetic mapping of genes makes possible the isolation of genes of agronomic importance through positional cloning, which requires a large-insert DNA library. Therefore, the large-insert DNA libraries, such as YAC, PAC, BAC, BIBAC, TAC, have been developed and utilized in many species. Sunflower (Helianthus annuus L.) is one of the world leading edible oilseed crops, and numerous genomic resources for sunflower have been developed, including various genetic maps and a large number of molecular markers, and several BAC libraries. To facilitate sunflower genomics research and develop molecular cytogenetic markers, we constructed two BAC and BIBAC libraries, and identified a set of sunflower linkage group-specific BAC or BIBAC clones from the libraries. These linkage group-specific clones will eventually assist to assign each sunflower chromosome to its corresponding linkage group.
Technical Abstract: One BAC library and one BIBAC library from an inbred line HA 89 were constructed by using two restriction enzymes (BamH1, HindIII) and two vectors (pECBAC1, pCLD04541). Using the large-insert libraries, we identified a set of sunflower linkage group-specific BAC or BIBAC clones by overgo hybridization. To characterize the quantity of the libraries, the amount of clones that originated from organellar DNA and possessed highly repetitive DNA sequences were estimated by screened the library with chloroplast or mitochondrial genome-specific genes, or Cot-1 DNA, respectively. These BAC and BIBAC libraries will provide resources essential for more comprehensive sunflower genomics research.