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Title: ECTOPIC EXPRESSION OF SINGLE-CHAIN ANTIBODIES AND EVALUATION OF RESISTANCE TO CUCUMBER MOSAIC VIRUS

Author
item Hammond, Rosemarie
item AEBIG, J - NIH/NIAID/MVDU
item Hsu, Hei Ti

Submitted to: APS Annual Meeting
Publication Type: Abstract Only
Publication Acceptance Date: 5/18/2006
Publication Date: 5/18/2006
Citation: Hammond, R., Aebig, J.A., Hsu, H. 2006. Ectopic expression of single-chain antibodies and evaluation of resistance to cucumber mosaic virus. [abstract]. APS Annual Meeting. 96:S45.

Interpretive Summary:

Technical Abstract: Diseases caused by the wide-host range Cucumber mosaic virus (CMV) can be devastating in field situations. One approach towards developing virus resistance or attenuating viral infection is by expression of recombinant antibodies in plants. We are using a plant virus episomal vector based on Potato virus X to transiently express 3 single-chain antibody (scFv) fragments derived from hybridoma cell lines secreting monoclonal antibodies (Mabs) with differential specificity to isolates of CMV. It had previously been shown that some of these Mabs neutralize virus infectivity and block virus transmission by aphids, making them candidates for engineering CMV resistance in plants. We will compare our results with those of prior experiments where transgenic Nicotiana benthamiana plants expressing the scFv fragments exhibited variable degrees of disease resistance upon virus challenge.