Submitted to: American Society of Plant Biologists
Publication Type: Abstract Only
Publication Acceptance Date: 4/4/2006
Publication Date: 8/7/2006
Citation: Chen, M., Yu, F., Frantz, J., Gray, J., Heckathorn, S. 2006. Protein profile changes of arabidopsis leaves in response to boron stress. American Society of Plant Biologists, Boston, MA. p. 159. Interpretive Summary:
Technical Abstract: Boron (B) stress (both deficiency and toxicity) is common in plants, but as the functions of this essential micronutrient are incompletely understood, the mechanisms of B stress are poorly understood too. To investigate the metabolic mechanisms underlying B-stress, we examined protein expression patterns of leaves of 31-day-old Arabidopsis thaliana plants grown under normal B (30µM), compared to plants transferred for 3 days to deficient (0µM) or toxic (3mM) levels of boron. B-responsive polypeptides were sequenced by mass spectrometry, following 1D or 2D gel electrophoresis, and immunoblotting was used to confirm the B-responsiveness of several of these proteins. Ten B-responsive proteins were identified, including three photosynthetic and two regulatory proteins, as well as five diverse “stress” proteins. Our results indicate multiple early effects of B-stress, including specific effects on photosynthesis, and identify proteins for use as early biomarkers for B stress.