Submitted to: American Society of Plant Biologists Annual Meeting
Publication Type: Abstract Only
Publication Acceptance Date: 3/10/2006
Publication Date: 8/5/2006
Citation: Yang, L., Lin, H., Fritschi, F.B., Walker, A. 2006. Identification and characterization of proteomic expression of grapevines in response to xylella fastidiosa. American Society of Plant Biologists Annual Meeting 2006. p. 174. Interpretive Summary:
Technical Abstract: Xylella fastidiosa (Xf) is the pathogen causing grape Pierce’s disease (PD) as well as economically important diseases in a number of fruit and ornamental plants. In this study, we developed a comparative proteomic analysis approach to characterize differentially expressed protein profiles from grapevines infected with Xf. Highly susceptible (9621-94) and highly resistant (9621-67) genotypes, sibling plants from a segregating Vitis rupestris x V. arizonica population were used for protein expression analysis. Proteins were isolated from stem, leaf and xylem sap samples of control and Xf-infected grapes at 1, 6 and 10 weeks post-inoculation. Proteins were separated by 2D-PAGE. Spots representing differential expression among resistant and susceptible plants and Xf-infected and control plants were identified with PDQuest software and excised with the ProteomeWorks Spot Cutter (BioRad). Isolated proteins were analyzed by an oMALDI-TOF-MS/MS Mass Spectrometry. Comparison analyses were also made between proteomic expression profiles and microarray gene expression derived from our EST project. Transcriptional and translational gene expressions profiles provide complementary details of the molecular events involved in the interactions between pathogen and host in signal recognition, signal transduction, pathogenesis and host defense responses between PD susceptible and resistant grapes during the disease development. Characterization and identification of differentially expressed proteins will promote identification and understanding of the underlying metabolic pathways that are involved in the mechanisms of PD resistance and susceptibility in grapes.