Submitted to: Euphytica
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 8/17/2005
Publication Date: 12/1/2005
Citation: Jia, G., Chen, P., Qui, G., Bai, G., Wang, X., Wang, S., Zhou, B., Zhang, S., Liu, D. 2005. Qtls for fusarium head blight response in a wheat dh population of wangshuibai/ alondra's'. Euphytica 146:183-191 Interpretive Summary: Fusarium head blight (FHB) causes serious losses in production of wheat worldwide. Wangshuibai is a highly resistant Chinese cultivar. It was crossed with Alondra’s’, which is a susceptible line, to make a mapping population. Progeny were tested for resistance in the field in China. Resistance genes were detected on wheat chromosomes 3B, 5B, 2D, 6A, and 7A. Markers associated with these genes could be useful for marker-assisted selection.
Technical Abstract: A doubled haploid (DH) wheat population derived from the cross Wangshuibai/Alondra’s’ was developed through chromosome doubling of haploids generated by anther culture of hybrids. Fusarium head blight (FHB) was evaluated for three years from 2001 to 2003 in Jianyang, Fujian Province, China, where epidemics of FHB have been consistently severe. After 307 pairs of simple sequence repeat (SSR) primers were screened, 110 pairs were polymorphic between Wangshuibai and Alondra’s’, and used to construct a genetic linkage map for detection of quantitative trait loci (QTLs). A stable QTL for low FHB severity was detected on chromosome 3B over all three years, and QTLs on chromosomes 5B, 2D, and 7A were detected over two years. Additional QTLs on chromosomes 3A, 3D, 4B, 5A, 5D, 6B, and 7B showed marginal significance in only one year. Six QTLs were detected phenotypic data from three years were combined. In addition, significant additive-by-additive epistasis was detected for a QTL on 6A although its additive effects was not significant. Additive effects (A) and additive-by-additive epistasis (AA) explained a major portion of the phenotypic variation (76.5%) for FHB response. Xgwm533-3B and Xgwm335-5B were the closest markers to QTLs, and have potential to be used as selectable markers for marker-assisted selection (MAS) in wheat breeding programs.