Submitted to: American Chemical Society Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 2/10/2006
Publication Date: 9/10/2006
Citation: Qi, X., Shelver, W.L., Dong, Y., Zou, M. 2006. Microfludic immunochemical ractopamine analysis. American Chemical Society Abstracts, September 10-14, 2006, San Francisco, CA.
Technical Abstract: Biocompatible polystyrene microfluidic cover and base chips were made by injection molding and all micro-channels were characterized by a simple and rapid 3D profile scanning method. The surfaces of the micro-channels on the base chip were processed with plasma to be more hydrophilic. A microfluidic chip workstation with a LED as the light source for spectrometer was devised and acted as a miniaturized microplate reader. The ractopamine-BSA conjugate was pre-coated onto the surface of the chip micro-channels and determination of ractopamine was performed on the chip using competitive immunoassay; a preliminary 5 ng/ml detection sensitivity comparable to that of a conventional 96-well microtiter plate method was achieved. Further microfluidic optimization experiments were needed to improve the detection capability.