Submitted to: International Congress of Plant Molecular Biology
Publication Type: Proceedings
Publication Acceptance Date: 4/21/2006
Publication Date: 8/25/2006
Citation: Kindiger, B.K. 2006. Amplification of lolium ssp. microsatellites in poa ssp.International Congress of Plant Molecular Biology. POS-TUE-039.
Interpretive Summary: Comparing the genomes of potentially related grass species such as perennial ryegrass and Kentucky bluegrass, can presumably provide useful information for grass improvement projects and the identification of molecular markers that may be transferable and useful across species or genus classifications. Preliminary studies using molecular markers from perennial ryegrass in Kentucky bluegrass suggested a good potential for identifying regions of genome conservation. For this study, perennial and annual ryegrass microsatellite molecular markers were evaluated for their potential to identify complementary genome regions across eight bluegrass species. Results of the study suggest that none of the microsatellite motif regions present in perennial or annual ryegrass were identified in any of the bluegrass species studied. Though no complementary microsatellite motif regions were identified, DNA sequence comparisons did identify regions in ryegrass and bluegrass that exhibited high and low levels of genome similarity. The study concluded that the use of ryegrass microsatellite markers is not an effective approach for identifying complementary microsatellite motif sites in bluegrass or for the generation of bluegrass microsatellite molecular markers. The identification of non-microsatellite regions exhibiting cross-genus similarity suggests some level of genome conservation between ryegrass and bluegrass; however, the complexity of the polyploid bluegrass genome amplifies the difficulties for future comparative genomic studies of these species.
Technical Abstract: Cross-species amplification of forty-seven Lolium ssp. microsatellite primers were evaluated across eight Poa species or sub-species. Of these, eighteen Lolium SSR primer pairs generated one or more amplification products in one or more Poa ssp. Sequence evaluation of the amplification products indicated that none of the Lolium SSR primer pairs identified complementary microsatellite regions in any Poa ssp. Sequence comparison of amplification products exhibiting similar and differing molecular weights suggest a wide degree of sequence complementation. ClustalW comparisons of the Lolium and Poa amplification products identified similarity percentages ranging from a low of 50% to a high of 94%. Though no Poa ssp. SSR's were generated, several of the amplification products were polymorphic within and across the Poa ssp. and could be utilized as markers in Poa ssp. intergeneric hybrid studies.