|Goodwin, Stephen - Steve|
|Van Der Lee, T|
Submitted to: Fungal Genetics Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 2/28/2006
Publication Date: 4/10/2006
Citation: Goodwin, S.B., Dunkle, L.D., Churchill, A.C., Carlier, J., James, A., Souza, M.T., Crous, P., Roux, N., Van Der Lee, T.A., Waalwijk, C. 2006. Sequencing mycosphaerella and cercospora species will revolutionize the control of major global threats on wheat, banana and maize. [Abstract]. Fungal Genetics Conference Proceedings. p. 23.
Technical Abstract: MYCOSPHAERELLA is one of the largest genera of plant pathogenic fungi with more than 1,000 named species; a few species cause disease in humans and other vertebrates. The major MYCOSPHAERELLA plant pathogens include M. GRAMINICOLA of wheat and M. FIJIENSIS of banana, which require global annual fungicide inputs of $400 million and $2.5 billion, respectively. A joint project between the USDA-ARS/Purdue University and Plant Research International B.V. was initiated to sequence the genomes of both species, along with 40,000 ESTs from both M. FIJIENSIS and the related maize pathogen CERCOSPORA ZEAE-MAYDIS. The work was conducted through the Community Sequencing Program sponsored by the U.S. DOE-Joint Genome Institute. The initial goals of the projects are to: assemble 8x genomic shotgun sequences of M. GRAMINICOLA and M. FIJIENSIS; perform automated annotations of these genomic sequences and directed annotations using the ~80,000 ESTs from both MYCOSPHAERELLA species (the M. GRAMINICOLA set will be made available by Syngenta); and make these sequences available publicly through a series of linked web sites for comparative analyses. Currently, the 8x M. GRAMINICOLA sequence (518,271 traces) is available at NCBI. An initial assembly was made after the 4x sequencing was complete. The 289,742 reads were organized into 2962 contigs spanning 37.65 Mb. These contigs were assembled into 187 scaffolds covering 39.05 Mb, giving a revised genome size of 41.8 Mb, slightly larger than estimated previously. In addition, a draft mitochondrial assembly yielded a 43,962-base scaffold that appears to cover the complete genome. A community-wide annotation effort culminating in an annotation jamboree is anticipated for later during 2006 and will be open to all interested participants.