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Title: SUMMARY OF THE ANIMAL HOMOLOGUE SECTION OF HUMAN LEUKOCYTE DIFFERENTIATION ANTIGENS (HLDA8) WORKSHOP

Author
item SAALMULLER, ARMIN
item Lunney, Joan
item DAUBENBERGER, CLAUDIA
item DAVIS, WILLIAM
item FISCHER, UWE
item GOBEL, THOMAS
item GRIEBEL, PHIL
item HOLLEMWEGUER, ENOC
item LASCO, TODD
item MEISTER, RICHARD
item SCHUBERTH, HANS-JOACHIM
item SESTAK, KAROL
item SOPP, PAUL
item STEINBACH, FALKO
item WU, XIAO-WEI
item AASTED, BENT

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 3/1/2006
Publication Date: 5/23/2006
Citation: Saalmuller, A., Lunney, J.K., Daubenberger, C., Davis, W., Fischer, U., Gobel, T.W., Griebel, P., Hollemweguer, E., Lasco, T., Meister, R., Schuberth, H., Sestak, K., Sopp, P., Steinbach, F., Wu, X., Aasted, B. 2006. Summary of the animal homologue section of human leukocyte differentiation antigens (hlda8) workshop [abstract]. International Society of Analytical Cytology (ISAC) meeting in Quebec, Canada, May 2006.

Interpretive Summary:

Technical Abstract: Development of reagents against leukocyte differentiation antigens in veterinary immunology is slower compared to human and mice. Cross-reactivity studies with monoclonal antibodies (mAb) generated against human molecules represent an excellent approach for the detection of new reagents for the minor characterised species. 377 commercially available mAb from different companies were tested for their reactivity with cells from 16 species – including non-human primates, ruminants, swine, horse, carnivores, rabbit, guinea pig, chicken and fish. In a first round 182 mAb showed at least reactivity with one of the species described above. Most of the cross-reactivity was found against non-human primate leukocytes, but also species in evolution more distant from humans showed in some cases a clear staining pattern in flow cytometry (FCM). In a second round these FCM-results were confirmed by molecular analyses, by immunoprecipitation studies and analyses on transfectants. Interesting was the broad species-overlapping reactivity of mAb directed against CD9 (11 out of 16 species), CD11a (10/16), CD14 (13/16), CD18 (12/16), CD29 (12/16), and CD49d, indicating evolutionary highly conserved epitopes on these surface molecules. Our results suggest the suitability of crossreactive mAb for the animal model studies. Moreover, these findings contribute to our understanding of the evolution of the immune system. Supported by the Veterinary Immunology Committee of IUIS.